While Taylor-Fletcher has missed four matches, Holdsworth has been out for three.
Tonight's game could also be telling for Nathan Clarke, who lost his centre-back berth two games ago and is eager to stake his claim for a return.
Of Jackson's 23-strong squad, only Mirfin and long-term absentee Tom Clarke (knee) will be missing, and the boss intends to give as many players as possible a run-out.
The game against the UniBond League Division I side takes on added importance because of the Port Vale call-off, and Jackson added: "It forms an important part of the build-up to Scunthorpe."
Town slipped from sixth to ninth in League I after Saturday's 3-0 home setback against Brighton, and Jackson is demanding a major improvement against third-placed Scunthorpe, who won 4-1 at the Galpharm last season.
Avenue, bossed by former Wakefield-Emley assistant manager Phil Sharpe and including experienced Hade Edge-based midfielder Neil Redfearn, whose father Brian played league football for the original Bradford PA club, are fifth in their table.
Redfearn, who is due to make his 1,000th competitive career appearance in Saturday's FA Trophy tie at home to Solihull Borough, will play against Town.
But ex-Guiseley man Simon Parke and former Wakefield-Emley player Richard Chattoe are ruled out through work commitments and injury respectively.
"We like to play a passing game, and we're looking forward to doing it against a side who will respect that," said Sharpe.
"It will be a good experience for our younger players like Tom Greaves and Jamie Longley, and we're keen to give a good account of ourselves."
Town (from): Glennon, Rachubka, Holdsworth, Adams, Hudson, N Clarke, Brandon, Worthington, Abbott, Taylor-Fletcher, Schofield, Ahmed, McAliskey, McIntosh, McCombe, Beckett, Collins, Hardy, Young, Booth, Hand.