The 4-2 win over Huddersfield Town proved to be Beppe Sannino’s last as Watford manager after the Italian resigned with his side second in the Championship.
Sannino, whose side were victorious despite having defender Gabriel Tamas sent off with the score at 2-2, went into the clash with question marks over his future.
There were suggestions of player unrest, and the 57-year-old, who took over last December, said he had “gone as far as I can” in the Vicarage Road role.
He added: “Whoever will be in charge in the future will have everything ready for him to deliver the success that everyone at the club would wish for.”
A statement from the club read: “Watford FC wishes to thank Beppe Sannino for all that he has achieved while with the club and we wish him well for his future in football.”
Former Chievo and Palermo chief Sannino had never worked outside Italy when he succeeded Gianfranco Zola in the Hornets hot seat.
There have been three managerial departures in the Championship since the start of the season, with Mark Robins leaving Town and David Hockaday exiting at Leeds.
In addition, Tony Pulis has left Premier League Crystal Palace, who have since appointed former Town boss Neil Warnock for a second spell as manager.