Huddersfield Town’s winning run at Under 21 level ended with a 2-1 defeat at Birmingham City.
Frankie Bunn’s side, who have already clinched the Professional Development League II North title, had won their previous four.
And Flo Bojaj gave them a 10th-minute lead in their penultimate regular-season game at St Andrew’s.
But the young Blues hit back in the second half through Koby Arthur and Emmanuel Mbende.
City had needed keeper Jake Weaver to make saves from Jake Charles and Jack Boyle to stop Town doubling their advantage.
Town head to Coventry City on Monday (2.00) ahead of their fourth successive appearance in the play-offs.
Team: Tadhg Ryan; Jack Senior, William Boyle, Fraser Horsfall, Danny Kane; Regan Booty, Lewis O’Brien; Jake Charles, Flo Bojaj, Jack Boyle; Rekeil Pyke.
Unused subs: Ryan Schofield, Rarmani Edmonds-Green, Jamie Spencer, Luca Colville, Cedwyn Scott.